Fig. 5
From: High-titer production of 13R-manoyl oxide in metabolically engineered Saccharomyces cerevisiae
Production of 13R-MO by batch and fed-batch cultivation in a 5 L bioreactor. a Batch fermentation using LZJ7 in the 5 L bioreactor. YPD medium containing 40 g/L of glucose was used for the fermentation; fermentation was conducted at 30 °C with an airflow rate of 2 vvm, and the pH was automatically maintained at 5.5. b Fed-batch fermentation using LZJ7 in the 5 L bioreactor. The feed solution was added at 18 h, as indicated by the red arrow