Table 3 Shake flask l-DOPA production in engineered E. coli BL21 strains
From: Metabolic engineering of Escherichia coli BL21 (DE3) for de novo production of l-DOPA from d-glucose
Strain | Modification in the BL21 strain | OD600 | Tyrosine (mg/L) | l-DOPA (mg/L) |
|---|---|---|---|---|
E. coli BL21 (pRSFDuet-1-hpaBC) | 6.2 ± 0.1 | 324.0 ± 3.4 | 119.6 ± 1.4 | |
BL21 LP-1 | E. coli BL21–∆tyrR | 6.4 ± 0.1 | 274.9 ± 5.6 | 196.2 ± 1.2 |
BL21 LP-2 | E. coli BL21–∆tyrR–∆ptsG–∆crr | 6.2 ± 0.0 | 354.2 ± 21.5 | 259.8 ± 11.2 |
BL21 LP-3 | E. coli BL21–∆tyrR–∆ptsG–∆crr–PT7–galP–glk | 5.9 ± 0.1 | 362.1 ± 44.6 | 318.9 ± 12.3 |
BL21 LP-4 | E. coli BL21–∆tyrR–∆ptsG–∆crr–PT7–galP–glk–∆pheA | 5.8 ± 0.1 | 421.0 ± 6.4 | 411.7 ± 2.3 |
BL21 LP-5 | E. coli BL21–∆tyrR–∆ptsG–∆crr–PT7–galP–glk–∆pheA–PT7–aroGfbr–tyrAfbr | 5.8 ± 0.0 | 364.0 ± 6.1 | 432.0 ± 4.1 |
BL21 LP-6 | E. coli BL21–∆tyrR–∆ptsG–∆crr–PT7–galP–glk–∆pheA–PT7–aroGfbr–tyrAfbr–PT7–galp–glk–ppsA–tktA | 5.8 ± 0.0 | 392.3 ± 32.4 | 548.7 ± 10.2 |
BL21 LP-7 | E. coli BL21–∆tyrR–∆ptsG–∆crr–PT7–galP–glk–∆pheA–PT7–aroGfbr–tyrAfbr–PT7–galp–glk–ppsA–tktA–pykFA | 5.5 ± 0.2 | 461.7 ± 12.0 | 593.9 ± 18.4 |