Fig. 1From: Metabolic engineering of Escherichia coli BL21 (DE3) for de novo production of l-DOPA from d-glucoseModular expression of chromosomal genes in a pseudo-operon configuration. A four-gene pathway assembled in a pseudo-operon configuration with the engineered pETM6 ePathBrick vector. The pseudo-operon configuration was achieved by digesting the donor vector with restriction enzyme pairs AvrII/SalI and ligating it to the SpeI/SalI digested destination vectorBack to article page