Table 3 Strains and plasmids used in this study
Strains and plasmids | Description | Reference or source |
|---|---|---|
Bacteria | ||
 B. amyloliquefaciens NK-1 | LL3 derivative, ∆pMC1, ∆upp | [43] |
 B. amyloliquefaciens NK-∆LP | NK-1 derivative, ∆pgsBCA | [43] |
 B. subtilis168 | trpC2, containing a strong constitutive promoter P43 | This lab |
 B. amyloliquefaciens NK-Pbca | NK-1 derivative with plasmid pCB-Pbca | This work |
 B. amyloliquefaciens NK-A2cup | NK-1 derivative with plasmid pCB-A2cup | This work |
 B. amyloliquefaciens NK-BJ27up | NK-1 derivative with plasmid pCB-BJ27up | This work |
 B. amyloliquefaciens NK-C2up | NK-1 derivative with plasmid pCB-C2up | This work |
 B. amyloliquefaciens NK-PamyA | NK-1 derivative with plasmid pCB-PamyA | This work |
 B. amyloliquefaciens NK-P43 | NK-1 derivative with plasmid pCB-P43 | This work |
 B. amyloliquefaciens C2LP | ∆LP derivative, C2up promoter inserted into upstream of the itu operon | This work |
 B. amyloliquefaciens C-Q | C2LP derivative with plasmid pHT-degQ | This work |
 B. amyloliquefaciens C-U | C2LP derivative with plasmid pHT-degU | This work |
 B. amyloliquefaciens C-A | C2LP derivative with plasmid pHT-comA | This work |
 B. amyloliquefaciens C-P | C2LP derivative with plasmid pHT-sfp | This work |
 B. amyloliquefaciens C-E | C2LP derivative with plasmid pHT-yczE | This work |
 B. amyloliquefaciens C-R | C2LP derivative with plasmid pHT-glnR | This work |
 B. amyloliquefaciens C-aB | C2LP derivative with plasmid pHT-antiabrB | This work |
 B. amyloliquefaciens C-aY | C2LP derivative with plasmid pHT-anticodY | This work |
 E. coli DH5α | F−, ϕ80dlacZM1, (lacZYA-argF)U169, deoR, recA1, endA1, hsdR17 (rk−, mk+), phoA, supE44, λ−thi-1, gyrA96, relA1 | This lab |
 E. coli GM2163 | F−, ara-14 leuB6 thi-1 fhuA31 lacY1 tsx-78 galK2 galT22 supE44 hisG4 rpsL136 (Strr) xyl-5 mtl-1 dam13::Tn9 (Camr) dcm-6 mcrB1 hsdR2 mcrA | This lab |
Fungi | ||
 Alternaria alternate | Wild-type | This lab |
 Botrytis cinerea | Wild-type | This lab |
 Colletotrichum gloeosporioides | Wild-type | This lab |
 Fusarium oxysporum | Wild-type | This lab |
 Rhizoctonia solani | Wild-type | This lab |
Plasmid | ||
 pCB | Shuttle vector containing the β-galactosidase gene bgaB from B. stearothermophilus as a reporter gene, Ampr, Err | Xie et al. [44] |
 pKSU | pKSV7 derivative with upp gene expression cassette | This lab |
 pHT01 | Cmr; IPTG inducible expression vector for Bacillus | MoBiTec |
 pCB-Pbca | pCB derivative with the promoter Pbca | This work |
 pCB-A2cup | pCB derivative with the promoter A2cup | This work |
 pCB-BJ27up | pCB derivative with the promoter BJ27up | This work |
 pCB-C2up | pCB derivative with the promoter C2up | This work |
 pCB-PamyA | pCB derivative with the promoter PamyA | This work |
 pCB-P43 | pCB derivative with the promoter P43 | This work |
 pKSU-C2itu | pKSU derivative, a C2up promoter insertion vector | This work |
 pHT-degQ | pHT01 derivative with the gene degQ | This work |
 pHT-degU | pHT01 derivative with the gene degU | This work |
 pHT-comA | pHT01 derivative with the gene comA | This work |
 pHT-sfp | pHT01 derivative with the gene sfp | This work |
 pHT-yczE | pHT01 derivative with the gene yczE | This work |
 pHT-glnR | pHT01 derivative with the gene glnR | This work |
 pHT-antiabrB | pHT01 derivative with the gene anti-abrB sRNA sequence and hfq gene | This work |
 pHT-anticodY | pHT01 derivative with the gene anti-codY sRNA sequence and hfq gene | This work |