Table 2 Experiment design and results of the Box–Behnken central composite design
Run order | Factors (g/l) | Titer (mg/l) | |||
|---|---|---|---|---|---|
A | B | C | Experimental | Predicted | |
1 | 1 | 5 | 0.5 | 15.70 ± 4.21 | 15.11 |
2 | 13 | 5 | 0.5 | 52.87 ± 5.07 | 44.98 |
3 | 1 | 25 | 0.5 | 22.00 ± 3.14 | 29.88 |
4 | 13 | 25 | 0.5 | 51.58 ± 1.73 | 52.17 |
5 | 1 | 15 | 0.1 | 36.43 ± 0.28 | 34.91 |
6 | 13 | 15 | 0.1 | 54.03 ± 0.51 | 59.80 |
7 | 1 | 15 | 0.9 | 28.07 ± 2.02 | 22.30 |
8 | 13 | 15 | 0.9 | 48.05 ± 0.81 | 49.57 |
9 | 7 | 5 | 0.1 | 62.02 ± 3.64 | 64.14 |
10 | 7 | 25 | 0.1 | 72.06 ± 3.71 | 65.69 |
11 | 7 | 5 | 0.9 | 36.93 ± 6.77 | 43.29 |
12 | 7 | 25 | 0.9 | 65.81 ± 7.67 | 63.70 |
13 | 7 | 15 | 0.5 | 92.76 | 96.83 |
14 | 7 | 15 | 0.5 | 96.60 | 96.83 |
15 | 7 | 15 | 0.5 | 94.20 | 96.83 |
16 | 7 | 15 | 0.5 | 105.69 | 96.83 |
17 | 7 | 15 | 0.5 | 94.89 | 96.83 |