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Table 1 Observed molecular weights of OmpF peptide fragments digested with trypsin and measured by liquid chromatography-mass spectrometry

From: Construction of a bacterial surface display system based on outer membrane protein F

Fragment

Peptide sequence

Theoretical M.W. (Da)

Observed M.W. (Da)

Lot 1

Lot 2

Lot 3

1

YADVGSFNYGR

1248.541

1249.605

1249.596

1249.638

2

YDANNIYLAANYGETR

1846.849

1847.867

1847.877

1847.931

3

NYGVVYDALGYTDMLPEFGGDTAYSDDFFVGR

3553.566

3554.815

3554.632

3554.830

4

GETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK

3693.573

3693.868

 

3694.669