Fig. 7
From: Construction of a bacterial surface display system based on outer membrane protein F
Immunoelectron microscopy of recombinant bacteria. a Electron microscopy (EM) images of ER0808 cells, ER0808-prL8HB cells, ER2566-pF-HB cells and ER2566-pC-HB cells, as indicated by the gold-conjugated E6F6 (set A, top panel) at ×49,000 magnification. b EM images of ER0808 cells, ER0808-prL8L2 cells, ER2566-pF-L2 cells and ER2566-pC-L2 cells as indicated by gold-conjugated 14H6 (set b, top panel) at ×49,000 magnification. For clarity, close-up views of the top panels are shown; red boxes indicate the magnified regions. The curve of some images below is adjusted to better visualize the gold particles by Photoshop software. Number of gold particles on the bacterial surface presented in the close-up views was counted by Multi-point tool of ImageJ software (http://rsb.info.nih.gov/ij/), and is shown below the images as “N”