Fig. 2From: Enhanced extracellular expression of Bacillus stearothermophilus α-amylase in Bacillus subtilis through signal peptide optimization, chaperone overexpression and α-amylase mutant selectionScreening of α-amylases containing different signal peptides. a Transparent circles formed on a soluble starch plate by B. subtilis RIK1285 strains containing α-amylase variants with different signal peptides. b Alpha-amylase activities of the extracellular supernatants obtained using B. subtilis RIK1285 strains cultured in a triangular shake flask. Strains a, b, and c represent three B. subtilis RIK1285 variants that produce α-amylase with different signal peptides; d is a control strain. Error bars indicate the standard deviation from the mean of the three experimental data replicatesBack to article page