Table 1 Plasmids and strains used in the present study
Plasmids/strains | Relevant characteristics | Source or reference |
|---|---|---|
Plasmids | ||
pET32a | T7 promoter, f1 ori, Ampr | Novagen |
pCDFduet-amp | double T7 promoter, CloDE13 ori, Strr, Ampr | This study [21] |
pSE34 | ermE* promoter, pIJ101 ori, Thior | |
pET-SaOMT2-H | pET32a carrying the SaOMT2 gene fused with a C-terminal histidine-tag | This study |
pPTS | pET32a carrying Os4CL, VvSTS, and VvROMT genes | This study |
pNRG | pCDFduet-amp carrying Os4CL, PeCHS, and MtCHI genes | This study |
pNRG-SaOMT2 | pCDFduet-amp carrying Os4CL, PeCHS, MtCHI, and SaOMT2 genes | This study |
pAPG | pCDFduet-amp carrying Os4CL, PeCHS, MtCHI, and PcFNS genes | This study |
pAPG-SaOMT2 | pCDFduet-amp carrying Os4CL, PeCHS, MtCHI, PcFNS, and SaOMT2 genes | This study |
pSE-SaOMT2 | pSE34 carrying the SaOMT2 gene | This study |
pSE-SaOMT2-H | pSE34 carrying the SaOMT2 gene fused with a C-terminal histidine-tag | This study |
Strains | ||
E. coli BL21 (DE3) | F− ompT hsdSB(r −B m −B ) gal dcm lon (DE3) | Novagen |
DHS2001 | Streptomyces venezuelae Δ pikromycin polyketide synthase | [33] |
BL21/SaOMT2-H | E. coli BL21(DE3) strain harboring pET-SaOMT2-H | This study |
BL21/PTS | E. coli BL21(DE3) strain harboring pPTS | This study |
BL21/NRG | E. coli BL21(DE3) strain harboring pNRG | This study |
BL21/NRG-SaOMT2 | E. coli BL21(DE3) strain harboring pNRG-SaOMT2 | This study |
BL21/APG | E. coli BL21(DE3) strain harboring pAPG | This study |
BL21/APG-SaOMT2 | E. coli BL21(DE3) strain harboring pAPG-SaOMT2 | This study |
DHS2001/SaOMT2 | DHS2001 strain harboring pSE-SaOMT2 | This study |
DHS2001/SaOMT2-H | DHS2001 strain harboring pSE-SaOMT2-H | This study |