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Fig. 3 | Microbial Cell Factories

Fig. 3

From: High-yield production of multiple O-methylated phenylpropanoids by the engineered Escherichia coli–Streptomyces cocultivation system

Fig. 3

Analysis of in vitro methylation reaction catalyzed by recombinant SaOMT2. a SDS-PAGE of purified SaOMT2 expressed in S. venezuelae (svSaOMT2). Lane M shows the molecular weight marker with the indicated masses. b UPLC–qTOF–HR-MS chromatogram selected for m/z = 285.0757, 299.0914, and 313.1071 corresponding to genkwanin (P2), 5-hydroxy-7,4′-dimethoxyflavone (P3), and 5,7,4′-trimethoxyflavone (P4), respectively, converted from apigenin (P1) by SaOMT2 expressed in S. venezuelae. c SDS-PAGE of purified SaOMT2 expressed in E. coli (ecSaOMT2). d UPLC– qTOF–HR-MS chromatogram selected for m/z = 285.0757 and 299.0914 corresponding to genkwanin (P2) and 5-hydroxy-7,4′-dimethoxyflavone (P3), respectively, converted from apigenin (P1) by SaOMT2 expressed in E. coli

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Microbial Cell Factories

ISSN: 1475-2859

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