Table 1 Strains and plasmids used in this study

C. glutamicum strains

 DelAro⁴-4cl_Pc C7

C. glutamicum derivative with in-frame deletions of cg0344-47, cg0502, cg1226 and cg2625-40, harboring a chromosomally encoded codon-optimized 4cl_Pc gene coding for 4-coumarate:CoA ligase from Petroselinum crispum under control of the T7 promoter (Δcg0344-47::P_T7-4cl_Pc) and replacement of the native gltA promoter with the dapA promoter variant C7 (P_gltA::P_dapA-C7)

[15]

 Nor1 C7

DelAro⁴-4cl_Pc C7 strain harboring pMKEx2-pcs_AaCg

This work

 Nor2 C7

DelAro⁴-4cl_Pc C7 strain harboring pMKEx2-pcs_AaCg-short

This work

 Nor2 C7 mufasO_BC

Nor2 C7 derivative with mutated fasO binding site upstream of accBC

This work

 Nor2 C7 mufasO_D1

Nor2 C7 derivative with mutated fasO binding site upstream of accD1

This work

 Nor2 C7 mufasO_BCD1

Nor2 C7 derivative with mutated fasO binding site upstream of accBC and accD1

This work

 Nor2 C5 mufasO_BCD1

Nor2 C7 mufasO_BCD1 derivative with replacement of the dapA promoter variant C7 with the dapA promoter variant C5

This work

 Nor2 C5 mufasO_BCD1 P_O6-iolT1

Nor2 C5 mufasO_BCD1 derivative with two nucleotide exchanges in the iolT1 promoter at position -113 (A→G) and -112 (C→G) relative to the start codon

This work

 Nor2 C5 mufasO_BCD1 P_O6-iolT1 ∆pyc

Nor2 C5 mufasO_BCD1 P_O6-iolT1 derivative with in-frame deletion of pyc

This work

E. coli strains

 DH5α

F–Φ80 lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17

(rK–,mKþ)phoA supE44λ–thi-1 gyrA96relA1

Invitrogen (Karlsruhe, Germany)

Plasmids

 pK19mobsacB

kan^r; vector for allelic exchange in C. glutamicum (pK18, oriV_Ec, sacB, lacZα)

[52]

 pK19mobsacB-mufasO_BC

Vector for mutation of the fasO binding site upstream of accBC

This work

 pK19mobsacB-mufasO_D1

Vector for mutation of the fasO binding site upstream of accD1

This work

 pK19mobsacB-P_gltA::P_dapA-C5

Vector for exchanging the P_dapA-C7 variant upstream of gltA against the P_dapA-C5 variant

[28]

 pK19mobsacB-P_O6-iolT1

Vector for mutation of the iolT1 promoter

[29]

 pK19mobsacB-∆pyc

Vector for in-frame deletion of pyc

This work

 pMKEx2

kan^r; E. coli-C. glutamicum shuttle vector (lacI, P_T7, lacO1, pHM1519 ori_Cg; pACYC177 ori_Ec)

[24]

 pMKEx2-pcs_AaCg

pMKEx2 derivative with gene coding for pentaketide chromone synthase from Aloe arborescens (codon-optimized)

This work

 pMKEx2-pcs_AaCg-short

pMKEx2 derivative with truncated gene coding for pentaketide chromone synthase from Aloe arborescens (codon-optimized)

This work