Strains and plasmids | Relevant characteristic(s) | References |
---|---|---|
Strains | ||
 E. coli BL21 (DE3) | F− ompT gal dcm lon hsdSB (rB mB) λ(DE3) | Stratagene |
 C. glutamicum JL-6 (i.e., CICIM B1031) | C. glutamicum AECr SDr FPs Metl, derived from strain ATCC13032, deposited in China Information Center of Industrial Microbial (CICIM) | CICIM |
 C. glutamicum RG | Replacement of the natural gapA gene with the Ptac-gapC-rrnBT1T2 cassette in strain JL-6 chromosome | This work |
 C. glutamicum RGI (i.e., C. glutamicum RG/Ptac icdSm) | Replacement of the natural icdCg gene with the Ptac-icdSm-rrnBT1T2 cassette in strain RG chromosome | This work |
 C. glutamicum RG/PdapA-L1 icdSm | Replacement of the natural icdCg gene with the PdapA-L1-icdSm-rrnBT1T2 cassette in strain RG chromosome | This work |
 C. glutamicum RG/Ptac-M icdSm | Replacement of the natural icdCg gene with the Ptac-M-icdSm-rrnBT1T2 cassette in strain RG chromosome | This work |
 C. glutamicum RG/Ptuf icdSm | Replacement of the natural icdCg gene with the Ptuf-icdSm-rrnBT1T2 cassette in strain RG chromosome | This work |
 C. glutamicum RG/Psod icdSm | Replacement of the natural icdCg gene with the Psod-icdSm-rrnBT1T2 cassette in strain RG chromosome | This work |
Plasmids | ||
 pUC57/icdSm | The plasmid with the cassette of icdSm gene (including Ptac promoter, rrnBT1T2 terminator and icdSm operon) | Synthetic |
 pK18mobsacB | Integration vector | [40] |
 pK18mobsacB/∆gapA::gapC | Integration vector for replacement of the gapA gene by the Ptac-gapC-rrnBT1T2 cassette | [17] |
 pK18mobsacB/∆icdCg::icdSm | Integration vector for replacement of the icdCg gene by the Ptac-icdSm-rrnBT1T2 cassette | This work |
 pK18mobsacB-PdapA-L1 icdSm | Integration vector for replacement of the Ptac promoter of icdSm gene by the dapA-L1 promoter | This work |
 pK18mobsacB-Ptac-M icdSm | Integration vector for replacement of the Ptac promoter of icdSm gene by the tac-M promoter | This work |
 pK18mobsacB-Ptuf icdSm | Integration vector for replacement of the Ptac promoter of icdSm gene by the tuf promoter | This work |
 pK18mobsacB-Psod icdSm | Integration vector for replacement of the Ptac promoter of icdSm gene by the sod promoter | This work |