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Table 4 Strains and plasmids used in this study

From: Equilibrium of the intracellular redox state for improving cell growth and l-lysine yield of Corynebacterium glutamicum by optimal cofactor swapping

Strains and plasmids

Relevant characteristic(s)

References

Strains

 E. coli BL21 (DE3)

F− ompT gal dcm lon hsdSB (rB mB) λ(DE3)

Stratagene

 C. glutamicum JL-6 (i.e., CICIM B1031)

C. glutamicum AECr SDr FPs Metl, derived from strain ATCC13032, deposited in China Information Center of Industrial Microbial (CICIM)

CICIM

 C. glutamicum RG

Replacement of the natural gapA gene with the Ptac-gapC-rrnBT1T2 cassette in strain JL-6 chromosome

This work

 C. glutamicum RGI (i.e., C. glutamicum RG/Ptac icdSm)

Replacement of the natural icdCg gene with the Ptac-icdSm-rrnBT1T2 cassette in strain RG chromosome

This work

 C. glutamicum RG/PdapA-L1 icdSm

Replacement of the natural icdCg gene with the PdapA-L1-icdSm-rrnBT1T2 cassette in strain RG chromosome

This work

 C. glutamicum RG/Ptac-M icdSm

Replacement of the natural icdCg gene with the Ptac-M-icdSm-rrnBT1T2 cassette in strain RG chromosome

This work

 C. glutamicum RG/Ptuf icdSm

Replacement of the natural icdCg gene with the Ptuf-icdSm-rrnBT1T2 cassette in strain RG chromosome

This work

 C. glutamicum RG/Psod icdSm

Replacement of the natural icdCg gene with the Psod-icdSm-rrnBT1T2 cassette in strain RG chromosome

This work

Plasmids

 pUC57/icdSm

The plasmid with the cassette of icdSm gene (including Ptac promoter, rrnBT1T2 terminator and icdSm operon)

Synthetic

 pK18mobsacB

Integration vector

[40]

 pK18mobsacB/∆gapA::gapC

Integration vector for replacement of the gapA gene by the Ptac-gapC-rrnBT1T2 cassette

[17]

 pK18mobsacB/∆icdCg::icdSm

Integration vector for replacement of the icdCg gene by the Ptac-icdSm-rrnBT1T2 cassette

This work

 pK18mobsacB-PdapA-L1 icdSm

Integration vector for replacement of the Ptac promoter of icdSm gene by the dapA-L1 promoter

This work

 pK18mobsacB-Ptac-M icdSm

Integration vector for replacement of the Ptac promoter of icdSm gene by the tac-M promoter

This work

 pK18mobsacB-Ptuf icdSm

Integration vector for replacement of the Ptac promoter of icdSm gene by the tuf promoter

This work

 pK18mobsacB-Psod icdSm

Integration vector for replacement of the Ptac promoter of icdSm gene by the sod promoter

This work