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Table 3 The DCW, l-lysine production, by-product accumulation, intracellular NADH and NADPH level of modified glutamate dehydrogenase C. glutamicum strains

From: Equilibrium of the intracellular redox state for improving cell growth and l-lysine yield of Corynebacterium glutamicum by optimal cofactor swapping

C. glutamicum strains

NAD-IDH [mU (mg protein)−1]

DCW (g L−1)

l-Lysine (g L−1)

By-products (g L−1)

NADHa

NADPHa

l-Valine

l-Threonine

l-Glutamate

Lactate

Ethanol

RGI (RG/Ptac icdSm)

243.5 ± 21.6

52.5 ± 2.8

121.4 ± 4.8

4.7 ± 0.48

6.3 ± 0.38

7.2 ± 0.32

1.7 ± 0.24

1.0 ± 0.13

1.52 ± 0.11

2.15 ± 0.24

RG/PdapA-L1 icdSm

23.2 ± 1.9

39.6 ± 3.5

45.6 ± 3.7

6.1 ± 0.54

5.4 ± 0.33

3.7 ± 0.34

1.2 ± 0.13

0.6 ± 0.05

1.05 ± 0.07

2.17 ± 0.18

RG/Ptac-M icdSm

321.4 ± 32.8

64.2 ± 5.8

109.7 ± 2.4

4.5 ± 0.27

6.9 ± 0.65

9.1 ± 0.72

1.8 ± 0.08

1.3 ± 0.15

1.86 ± 0.14

2.08 ± 0.13

RG/Ptuf icdSm

509.4 ± 26.3

34.5 ± 3.7

39.2 ± 2.9

3.2 ± 0.23

4.3 ± 0.45

6.5 ± 0.58

2.1 ± 0.27

1.2 ± 0.08

2.47 ± 0.31

1.98 ± 0.16

RG/Psod icdSm

437.6 ± 34.2

49.6 ± 6.3

92.8 ± 8.3

4.3 ± 0.32

6.5 ± 0.48

7.9 ± 0.63

1.9 ± 0.14

1.1 ± 0.11

2.12 ± 0.11

1.93 ± 0.19

  1. All data are meaning values of three determinations of three independent experiments with ± SD
  2. aThe sampling time is 10 h, and the unit is μmol (g DCW)−1