Equilibrium of the intracellular redox state for improving cell growth and l-lysine yield of Corynebacterium glutamicum by optimal cofactor swapping

Table 1 In vitro specific activities of enzymes in genetically modified C. glutamicum strains and original strain C. glutamicum JL-6 as well as wild-type C. glutamicum ATCC13032

C. glutamicum strains	Specific activity [mU (mg protein)⁻¹]
	GAPDH		IDH
	NAD⁺	NADP⁺	NAD⁺	NADP⁺
JL-6	279.5 ± 12.5	–	4.5 ± 1.4	232.5 ± 19.8
RG	–	232.1 ± 15.7	8.9 ± 1.1	216.2 ± 27.3
RGI	–	253.1 ± 21.2	194.5 ± 21.6	–

The assay mixture contained 1 mmol L⁻¹ NAD⁺ or NADP⁺ as cofactor, and one unit of enzyme activity corresponds to 1 μmol NADH or NADPH formed per min, which was monitored continuously at A₃₄₀
All data are meaning values of three determinations of three independent experiments with ± SD
–, not detected

ISSN: 1475-2859