Fig. 1From: Metabolic engineering of Ashbya gossypii for deciphering the de novo biosynthesis of γ-lactonesMetabolic engineering of the de novo biosynthesis of lactones in A. gossypii. a Schematic diagram of the interconnection between the metabolic pathways involved in the de novo biosynthesis of lactones. An acyln−2-CoA represents a fatty acid from which two carbons of the initial chain length were removed after 1 cycle of β-oxidation. b Heterologous overexpression cassette constructed using the Golden-Gate rapid assembly. Grey-circled names represent the genes targeted for engineering. Dashed lines represent multi-reactions. M-CoA: malonyl-CoA; trans-2,3-DA-Coa: trans-2,3-dehydroacyl-CoABack to article page