Fig. 3

Dissecting the design criteria of engineering metabolite responsive transcriptional factors (MRTFs) in eukaryotic cells. a Engineering activator-based MRTF sensors in eukaryotic cells. b Engineering repressor-based MRTF sensors in eukaryotic cells. VP 16, VP 64, FapR, FdeR, PcaQ, ArgP, MdcR, Yap1 and Gal4 are a collection of transcriptional activators that are commonly used in eukaryotic cells. TetR, TrpR, FadR, PhlF, LexA and XylR are representative transcriptional repressors commonly used in eukaryotic cells. Core promoter or minimal promoter contain only the core TF binding sites and the TATA box