Fig. 4From: Construction of DGLA producing cell factory by genetic modification of Mucor circinelloidesStructure of plasmids pMAT1552 and pMAT1552-GLELO are shown. The D6E (GLELO) gene was isolated by PCR amplification with appropriate primers. The PCR fragment was ligated into XhoI restriction site to generate plasmid as pMAT1552-GLELOBack to article page