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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Optimizing a CRISPR-Cpf1-based genome engineering system for Corynebacterium glutamicum

Fig. 5

Multigene editing for isobutyrate production in C. glutamicum using the optimized CRISPR-Cpf1 system. a Schematic diagram of the central metabolic pathways and the introduced isobutyrate biosynthesis pathway in C. glutamicum. Gene knockout or gene expression is marked in red or green, respectively. PEP, phosphoenolpyruvate; KIV, 2-ketoisovalerate; OAA, oxaloacetate. b Isobutyrate production in a 60-h fermentation. ZJ0-0 represents wild-type (WT) C. glutamicum ATCC13032 with the empty vector, pKJ0; ZJ0-1 represents WT C. glutamicum ATCC13032 with pKJ1; ZJ1-1 represents WT∆pyc with pKJ1; ZJ2-1 represents WT∆ldh with pKJ1; ZJ3-1 represents WT∆adhA with pKJ1; ZJ12-1 represents WT∆pyldh with pKJ1; ZJ13-1 representsWT∆pycadhA with pKJ1; ZJ23-1 represents WT∆ldhadhA with pKJ1; ZJ123-1 represents WT∆pycldhadhA with pKJ1. Plasmid pKJ1 contains the isobutyrate biosynthetic pathway. c Cell growth in a 60-h fermentation

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