Fig. 4

Effects of repair template type on the CRISPR-Cpf1 system editing efficiencies in C. glutamicum. a–c Schematic overview of CRISPR-Cpf1 dependent repair of the same DSBs using plasmid-HA (a), pJET-HA (b) or linear-HA (c) as templates. d, e The editing colony number when targeting the upp gene (d) and editing efficiency when targeting the crtYe/f gene (e) using different repair template types. f PCR validation of upp gene deletion using different repair template types. The F17/R17 primers bind outside of the homologous arms. The amplified fragments are 2814 bp (wild-type) and 2128 bp (edited strain). g PCR validation of crtYe/f gene deletion using different repair template types. The F10/R10 primers bind outside of the homologous arms. The amplified fragments are 3025 bp (wild-type) and 2320 bp (edited strain)