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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Optimizing a CRISPR-Cpf1-based genome engineering system for Corynebacterium glutamicum

Fig. 3

On-target gene editing efficiencies of crRNAs with spacer sequences of different lengths. a Schematic diagram of crRNA spacer sequence optimization. be The effect of the length and sequence of the crRNA spacer regions on editing efficiencies of different genes. crRNA targets upp using the PAM sequences, 5′-TTTA-3′ (b) or 5′-CTTA-3′ (c). crRNA targets crtYe/f using the PAM sequences, 5′-TTTA-3′ (d) or 5′-CTTA-3′ (e)

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