Table 1 Kinetic parameters of SaDAE WT and V105A on four substrates
Enzymes | Substrates | Km (mM) | kcat (s−1) | kcat/Km (s−1 mM−1) | Relative activity (%) |
|---|---|---|---|---|---|
WT | d-Fructose | 59.3 ± 2.5 | 22.9 ± 0.49 | 0.39 | 49.85 ± 1.36 |
d-Allulose | 41.5 ± 1.8 | 44.6 ± 0.9 | 1.1 | 100 ± 3.2 | |
d-Tagatose | 107 ± 3.5 | 17.2 ± 0.65 | 0.16 | 39 ± 1.0 | |
d-Sorbose | 363 ± 10.2 | 4.4 ± 0.09 | 0.01 | 8 ± 0.2 | |
V105A | d-Fructose | 37.79 ± 2.1 | 54.93 ± 1.6 | 1.45 | 112.78 ± 5.1 |
d-Allulose | 38.85 ± 2.8 | 45.46 ± 3.2 | 1.17 | 102.59 ± 4.6 | |
d-Tagatose | 39.55 ± 2.1 | 43.32 ± 0.8 | 1.09 | 95.10 ± 3.2 | |
d-Sorbose | 150.19 ± 6.1 | 18.09 ± 0.5 | 0.126 | 24.47 ± 1.3 |