Skip to main content

Table 1 Bacterial strains and plasmids used in this study

From: Precise tuning of the glyoxylate cycle in Escherichia coli for efficient tyrosine production from acetate

Name

Description

Source

Strains

 E. coli Mach1-T1R

Cloning host

Invitrogen

 SCK1

W3110 ΔtyrR aroG:: PBBa_J23100-synUTRaroG-aroGfbr tyrA:: PBBa_J23100-synUTRtyrA-tyrAfbr ParoABCDELtyrB-UTRaroABCDELtyrB:: PBBa_J23100-synUTRaroABCDELtyrB

[17]

 SCKE

SCK1/pACYCduet-1

This study

 SCKA

SCK1/pACA

This study

 SCKP

SCK1/pACP

This study

 SCKAP

SCK1/pACAP

This study

 SCKDIAP

SCK1 ΔiclR::FRT-KmR-FRT/pACAP

 

 SCKAPG1

SCK1/pACAPG1

This study

 SCKAPG2

SCK1/pACAPG2

This study

 SCKAPG3

SCK1/pACAPG3

This study

 SCKAPG4

SCK1/pACAPG4

This study

 SCKAPG5

SCK1/pACAPG5

This study

Plasmids

 pKD46

Red recombinase expression vector, AmpR

[40]

 pM_FKF

PCR template for FRT-KanR-FRT, pMB1 ori, AmpR, KmR

[12]

 pACYCduet-1

p15A ori, CmR, E. coli expression vector

Novagen

 pACA

p15A ori, CmR, PBBa_J23100-synUTRacs-acs-TerBBa_B1006

This study

 pACP

p15A ori, CmR, PBBa_J23100-synUTRpck-pck-TerBBa_B1006

This study

 pACAP

p15A ori, CmR, PBBa_J23100-synUTRacs-acs-TerBBa_B1006-PBBa_J23100-synUTRpck-pck-TerBBa_B1006

This study

 pACAPG1

p15A ori, CmR, PBBa_J23100-synUTRacs-acs-TerBBa_B1006-PBBa_J23100-synUTRpck-pck-TerBBa_B1006-PBBa_J23104-synUTRaceA-aceA-TerBBa_B1006

This study

 pACAPG2

p15A ori, CmR, PBBa_J23100-synUTRacs-acs-TerBBa_B1006-PBBa_J23100-synUTRpck-pck-TerBBa_B1006-PBBa_J23118-synUTRaceA-aceA- TerBBa_B1006

This study

 pACAPG3

p15A ori, CmR, PBBa_J23100-synUTRacs-acs-TerBBa_B1006-PBBa_J23100-synUTRpck-pck-TerBBa_B1006-PBBa_J23116-synUTRaceA-aceA-TerBBa_B1006

This study

 pACAPG4

p15A ori, CmR, PBBa_J23100-synUTRacs-acs-TerBBa_B1006-PBBa_J23100-synUTRpck-pck-TerBBa_B1006-PBBa_J23109-synUTRaceA-aceA- TerBBa_B1006

This study

 pACAPG5

p15A ori, CmR, PBBa_J23100-synUTRacs-acs-TerBBa_B1006-PBBa_J23100-synUTRpck-pck-TerBBa_B1006-PBBa_J23100-synUTRaceA-aceA-TerBBa_B1006

This study

  1. Amp ampicillin, Cm chloramphenicol, Km kanamycin; R resistance