Name | Description | Source |
---|---|---|
Strains | ||
 E. coli Mach1-T1R | Cloning host | Invitrogen |
 SCK1 | W3110 ΔtyrR aroG:: PBBa_J23100-synUTRaroG-aroGfbr tyrA:: PBBa_J23100-synUTRtyrA-tyrAfbr ParoABCDELtyrB-UTRaroABCDELtyrB:: PBBa_J23100-synUTRaroABCDELtyrB | [17] |
 SCKE | SCK1/pACYCduet-1 | This study |
 SCKA | SCK1/pACA | This study |
 SCKP | SCK1/pACP | This study |
 SCKAP | SCK1/pACAP | This study |
 SCKDIAP | SCK1 ΔiclR::FRT-KmR-FRT/pACAP |  |
 SCKAPG1 | SCK1/pACAPG1 | This study |
 SCKAPG2 | SCK1/pACAPG2 | This study |
 SCKAPG3 | SCK1/pACAPG3 | This study |
 SCKAPG4 | SCK1/pACAPG4 | This study |
 SCKAPG5 | SCK1/pACAPG5 | This study |
Plasmids | ||
 pKD46 | Red recombinase expression vector, AmpR | [40] |
 pM_FKF | PCR template for FRT-KanR-FRT, pMB1 ori, AmpR, KmR | [12] |
 pACYCduet-1 | p15A ori, CmR, E. coli expression vector | Novagen |
 pACA | p15A ori, CmR, PBBa_J23100-synUTRacs-acs-TerBBa_B1006 | This study |
 pACP | p15A ori, CmR, PBBa_J23100-synUTRpck-pck-TerBBa_B1006 | This study |
 pACAP | p15A ori, CmR, PBBa_J23100-synUTRacs-acs-TerBBa_B1006-PBBa_J23100-synUTRpck-pck-TerBBa_B1006 | This study |
 pACAPG1 | p15A ori, CmR, PBBa_J23100-synUTRacs-acs-TerBBa_B1006-PBBa_J23100-synUTRpck-pck-TerBBa_B1006-PBBa_J23104-synUTRaceA-aceA-TerBBa_B1006 | This study |
 pACAPG2 | p15A ori, CmR, PBBa_J23100-synUTRacs-acs-TerBBa_B1006-PBBa_J23100-synUTRpck-pck-TerBBa_B1006-PBBa_J23118-synUTRaceA-aceA- TerBBa_B1006 | This study |
 pACAPG3 | p15A ori, CmR, PBBa_J23100-synUTRacs-acs-TerBBa_B1006-PBBa_J23100-synUTRpck-pck-TerBBa_B1006-PBBa_J23116-synUTRaceA-aceA-TerBBa_B1006 | This study |
 pACAPG4 | p15A ori, CmR, PBBa_J23100-synUTRacs-acs-TerBBa_B1006-PBBa_J23100-synUTRpck-pck-TerBBa_B1006-PBBa_J23109-synUTRaceA-aceA- TerBBa_B1006 | This study |
 pACAPG5 | p15A ori, CmR, PBBa_J23100-synUTRacs-acs-TerBBa_B1006-PBBa_J23100-synUTRpck-pck-TerBBa_B1006-PBBa_J23100-synUTRaceA-aceA-TerBBa_B1006 | This study |