Fig. 4

Effect of glyoxylate cycle activation. Comparison of normalized specific isocitrate lyase activity measured at 12 h (a), and cell biomass (b) and tyrosine production (c) after 30 h cultivation, and the fermentation profile of the SCKAPG4 strain (d). The left y-axis shows OD₆₀₀ and the right y-axis indicates the concentration of accumulated tyrosine; the y-offset indicates the concentration of the remaining acetate. The x-axis denotes time. Symbols: black circle, cell biomass (OD₆₀₀); red squares, tyrosine; blue diamonds, acetate. Error bars indicate the standard deviation from three independent cultures