Fig. 2

Generation of N. oleoabundans transformants. a Schematic map of plasmids pAR-LPAAT and pB2-LPAAT used for electroporation in N. oleoabundans. The NeoLPAAT1 cDNA (GenBank accession no.: MF706164) [21] was expressed under the control of either promoter AR (HSP70-RBCS2) [44] or β2-Tub (β2-tubulin) [45] and harbored 3′rbcS2 [47] at 3′end. Hyg3 gene, used as selectable marker [20, 46]. The 943-bp PCR amplicon, denoted by a line. b Genomic PCR detection of transformants harboring the NeoLPAAT1-expression cassettes. Genomic PCR of transformants AR-LPAAT, B2-LPAAT and wild type was performed with primers specifically bind to NeoLPAAT1 coding sequence. The 943-bp amplicon was detected in the transformants but not in wild type (used as negative control). Lanes M, 100-bp DNA ladder; C+ , plasmid pAR-LPAAT (used as positive control)