Fig. 1
Comparison of NeoLPAAT1 with other plastidial LPAAT1. a Sequence alignment of CrLPAAT1, AtLPAAT1 (ATS2) and NeoLPAAT1. The amino acid sequences of CrLPAAT1 (Chlamydomonas reinhardtii, Uniprot: A8J0J0), AtLPAAT1 (Arabidopsis thaliana, Uniprot: Q8GXU8) and NeoLPAAT1 (N. oleoabundans, GenBank protein id: AUS8446) were aligned using ClustalW algorithm [22]. The chloroplast transit peptides of CrLPAAT1, AtLPAAT1 [15] and NeoLPAAT1 predicted using PredAlgo [25] are highlighted in red. Membrane-spanning domains predicted by TMHMM [24] are highlighted in yellow. Conserved and similar residues are indicated by black and gray boxes, respectively. The conserved motifs I, II, III and IV [23] are indicated. The highly conserved residues in the motif are indicated by asterisks. b Phylogenetic analysis of NeoLPAAT1. The phylogenetic tree was constructed using MEGA 7 [26]. The percentage of neighbor-joining boot strap replications is shown above each node. Scale bar indicates 0.1 amino acid substitutions per site. Green algae: NeoLPAAT1 (N. oleoabundans, GenBank protein id: AUS8446), CvLPAAT1 (Chlorella variabilis, Uniprot: E1ZQN6), CrLPAAT1 (C. reinhardtii, Uniprot: A8J0J0), VcLPAAT1 (Volvox carteri, Uniprot: D8U1V6) and OlLPAAT1 (Ostreococcus lucimarinus, Uniprot: A4S0H0). Red alga: CmLPAAT1 (Cyanidioschyzon merolae, Uniprot: M1V4N2). Diatom: PtLPAAT1 (Phaeodactylum tricornutum, Uniprot: B7FQL9). Plants: AtLPAAT1 (A. thaliana, Uniprot: Q8GXU8) and BnLPAAT1 (Brassica napus, Uniprot: Q9LLY4)