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Table 3 Cultivation parameters of S. cerevisiae ST938 grown in fed-batch mode in 1-L bench-top bioreactors under carbon (C) and phosphorus (P) limiting conditions

From: Exploring small-scale chemostats to scale up microbial processes: 3-hydroxypropionic acid production in S. cerevisiae

 

Fed-batch

C-limitation

P-limitation

M (h−1)

0.059

0.051

YX/S (g mol−1)

77.9 ± 8.9

67.7 ± 6.4

YEtOH/S (mol mol−1)

b.d.

b.d.

YGly/S (mol mol−1)

b.d.

b.d.

Y3-HP/S (%C-mol)

15.9 ± 1.2

25.6 ± 1.7

Y3-HP/X (g gCDW−1)

0.38 ± 0.02

0.65 ± 0.09

qS (mmol gCDW−1 h−1)

0.75 ± 0.10

0.77 ± 0.05

q3-HP (mmol gCDW−1 h−1)

0.24 ± 0.00

0.39 ± 0.01

qCO2 (mmol gCDW−1 h−1)

1.94 ± 0.48

1.88 ± 0.16

Carbon balance (%)

105 ± 4.3

100 ± 4.2

  1. The growth rate (µset) of the exponential feeding profile was 0.05 h−1. Errors correspond to the standard deviations derived from triplicate cultivations
  2. X biomass, S substrate (glucose), EtOH ethanol, Gly glycerin, 3-HP 3-hydroxypropionic acid, b.d. below detection limit