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Table 1 Cultivation parameters of S. cerevisiae ST938 grown in continuous cultures under C-limited conditions

From: Exploring small-scale chemostats to scale up microbial processes: 3-hydroxypropionic acid production in S. cerevisiae

 

Chemostat

Batch

Dilution rate (h−1)

µmax (h−1)

0.04

0.09

0.17

0.21

0.27

YX/S (g mol−1)

78.4 ± 0.6

79.7 ± 5.8

90.3 ± 6.6

102.4 ± 0.7

24.9 ± 1.3

YEtOH/S (mol mol−1)

0.09 ± 0.03

0.04 ± 0.02

0.04 ± 0.01

0.04 ± 0.00

1.1 ± 0.0

YGly/S (mol mol−1)

0.03 ± 0.01

0.02 ± 0.00

0.02 ± 0.00

0.00 ± 0.00

0.1 ± 0.0

Y3-HP/S (mol mol−1)

0.33 ± 0.03

0.30 ± 0.00

0.26 ± 0.00

0.23 ± 0.00

0.01 ± 0.00

Y3-HP/S (%C-mol)

16.6 ± 1.7

15.1 ± 0.4

13.1 ± 0.4

11.7 ± 1.5

0.6 ± 0.0

Y3-HP/X (g gCDW−1)

0.43 ± 0.04

0.34 ± 0.03

0.26 ± 0.01

0.19 ± 0.02

0.04 ± 0.00

qS (mmol gCDW−1 h−1)

0.51 ± 0.13

1.2 ± 0.1

1.9 ± 0.2

2.1 ± 0.0

10.7 ± 0.7

qGly (mmol gCDW−1 h−1)

0.01 ± 0.00

0.02 ± 0.00

0.03 ± 0.00

0.06 ± 0.01

0.86 ± 0.03

qEtOH (mmol gCDW−1 h−1)

0.05 ± 0.02

0.05 ± 0.02

0.07 ± 0.02

0.08 ± 0.01

11.9 ± 0.5

q3-HP (mmol gCDW−1 h−1)

0.17 ± 0.06

0.36 ± 0.03

0.50 ± 0.03

0.49 ± 0.07

0.12 ± 0.00

Glcres (mM)

0.07 ± 0.00

0.08 ± 0.01

0.06 ± 0.03

0.05 ± 0.02

n.a.

  1. Errors correspond to standard deviations derived from triplicate cultivations
  2. Y yield, q rate, X biomass, S substrate (glucose), EtOH ethanol, Gly glycerin, 3-HP 3-hydroxypropionic acid, Glcres residual glucose, n.a. not applicable