Table 3 Effects of the overexpression of heme biosynthetic genes on heme accumulation and chromophorylation ratio of SLA-PEB
From: Metabolic engineering of Escherichia coli for efficient biosynthesis of fluorescent phycobiliprotein
E. coli strain | Expressed heme gene(s) | Heme (μmol/L) | Chromophorylation ratio (%) |
|---|---|---|---|
SLA-V4B | – | 1.94 ± 0.16 | 83.6 ± 4.92 |
V5AL | hemA, hemL | 2.96 ± 0.26 | 94.7 ± 3.23 |
V5ALB | hemA, hemL, hemB | 1.95 ± 0.17 | 85.3 ± 2.15 |
V5ALD | hemA, hemL, hemD | 3.30 ± 0.24 | 98.6 ± 3.98 |
V5ALF | hemA, hemL, hemF | 2.78 ± 0.44 | 89.3 ± 3.53 |
V5ALG | hemA, hemL, hemG | 2.36 ± 0.18 | 87.4 ± 2.70 |
V5ALH | hemA, hemL, hemH | 1.70 ± 0.19 | 85.2 ± 4.12 |