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Table 2 DNA and amino acid changes in the CpcS mutants and chromophorylation ratios of SLA-PEB purified from the wild type and mutant strains

From: Metabolic engineering of Escherichia coli for efficient biosynthesis of fluorescent phycobiliprotein

Strains

CpcS mutants

Base changes

Residual changes

Chromophorylation ratio (%)

SLA-V1

CpcS

16.2 ± 1.2

SLA-V2

CpcS

38.6 ± 4.6

SLA-V3-2

CpcSM2

T100C, A125G

S34P, E42G

56.2 ± 6.0

SLA-V3-7

CpcSM7

A302G, A460T

E101G, A177P

64.7 ± 3.2

SLA-V3-9

CpcSM9

T30C, A167G, A302G

Q56R, E101G

46.9 ± 5.5

SLA-V3-12

CpcSM12

A287G, C316T, T410C

Q96R, I137T

61.4 ± 5.8

SLA-V3-16

CpcSM16

T127C, T172C, A221G

Y58H, D74G

53.6 ± 2.7

  1. Data represent mean ± SD from three independent experiments