Table 1 Plasmids used in this study
From: Metabolic engineering of Escherichia coli for efficient biosynthesis of fluorescent phycobiliprotein
Plasmids | Description | Source |
|---|---|---|
pRSFDuet-1 | Double T7 promoters, RSF ori, KmR | Novagen |
pCDFDuet-1 | Double T7 promoters, P15A ori, CmR | Novagen |
pRSF-PHo1-pebS | pRSFDuet-1 containing PHo1 and pebS | Wu et al. [17] |
pCDF-SLA-cpcS | pCDFDuet-1 containing SLA and cpcS | Wu et al. [17] |
pRSF-SLA-cpcS-PHo1-pebS | pRSFDuet-1 containing SLA and apcA, cpcS, PHo1 and pebS | This study |
pRSF-SLA-cpcSM7-BHo1-pebS | pRSFDuet-1 containing SLA and apcA, cpcSM7, BHo1 and pebS | This study |
pRSF-SLA-cpcSM7-6Ho1-pebS | pRSFDuet-1 containing SLA and apcA, cpcSM7, 6Ho1 and pebS | This study |
pRSF-SLA-cpcSM7-7Ho1-pebS | pRSFDuet-1 containing SLA, cpcSM7, 7Ho1 and pebS | This study |
pRSF-SLA-cpcSM7-9Ho1-pebS | pRSFDuet-1 containing SLA, cpcSM7, 9Ho1 and pebS | This study |
pRSF-SLA-cpcSM7-PHo1-pebS | pRSFDuet-1 containing SLA, cpcSM7, PHo1 and pebS | This study |
pRSF-PHo1 | pRSFDuet-1 containing PHo1 | This study |
pRSF-6Ho1 | pRSFDuet-1 containing 6Ho1 | This study |
pRSF-7Ho1 | pRSFDuet-1 containing 7Ho1 | This study |
pRSF-9Ho1 | pRSFDuet-1 containing 9Ho1 | This study |
pRSF-BHo1 | pRSFDuet-1 containing BHo1 | This study |
pRSF-6Ho1-pebS | pRSFDuet-1 containing 6Ho1 and pebS | This study |
pRSF-7Ho1-pebS | pRSFDuet-1 containing 7Ho1 and pebS | This study |
pRSF-9Ho1-pebS | pRSFDuet-1 containing 9Ho1 and pebS | This study |
pRSF-BHo1-pebS | pRSFDuet-1 containing BHo1and pebS | This study |
pKD46 | repA101ts and oriR101 ParaB exo, bet, gam araC bla | Datsenko and Wanner [22] |
pKIKOarsBCm | R6K ori, CmR | Sabri et al. [21] |
pKIKOarsB-hemLA | pKIKOarsBCm containing expression cassette of hemLA | This study |
pKIKOarsB-hemLAB | pKIKOarsBCm containing expression cassette of hemLAB | This study |
pKIKOarsB-hemLAD | pKIKOarsBCm containing expression cassette of hemLAD | This study |
pKIKOarsB-hemLAF | pKIKOarsBCm containing expression cassette of hemLAF | This study |
pKIKOarsB-hemLAG | pKIKOarsBCm containing expression cassette of hemLAG | This study |
pKIKOarsB-hemLAH | pKIKOarsBCm containing expression cassette of hemLAH | This study |