Fig. 1
From: Metabolic engineering of Escherichia coli for efficient biosynthesis of fluorescent phycobiliprotein
Schematic presentation of strategy for SLA-PEB biosynthesis in E. coli. a Pathway for SLA-PEB biosynthesis. The pathway for SLA-PEB is divided into two modules: the endogenous heme pathway and the heterologous SLA pathway. GSA glutamate-1-semialdehyde, ALA 5-aminolevulinic acid, PBG porphobilinogen, HMB hydroxymethylbilane, HemA glutamyl-tRNA reductase, HemL glutamate-1-semialdehyde aminotransferase, HemB 5-aminolevulinic acid dehydratase, HemC porphobilinogen deaminase, HemD uroporphyrinogen III synthase, HemE uroporphyrinogen decarboxylase, HemF coproporphyrinogen III oxidase, HemG protoporphyrin oxidase, HemH ferrochelatase, Ho1 heme oxygenase 1, pebS phycoerythrobilin synthase, CpcS lyase. b Plasmid construction: the four genes required for SLA-PEB biosynthesis were cloned into the plasmids pCDFDuet-1 and pRSFDuet-1 or into a single plasmid pRSFDuet-1