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Table 1 Summary of monoterpene synthase that have been tested for monoterpene production in R. toruloides

From: Monoterpene production by the carotenogenic yeast Rhodosporidium toruloides

Product

Gene name

Organism

Gene bank

Access number

Enzyme kinetic for GPP

Product in R. toruloides

References

Dodecane overlay

SPME

1,8-Cineole

Hyp3

Hypoxylon sp. E74060B

KJ433271.1

Km = 2.5 ± 0.6 µM

Kcat = 0.295 S−1

+++

+++

[58]

SSCG_00536

CnsA

Streptomyces clavuligerus

DS570626.1

Km = 0.17 µM

Kcat = 0.079 S−1

++

++

[59]

Ocimene

ama0e23

Antirrhinum majus

AY195607.1

NA

ND

++

[60]

LcTPS1

Licea cubeba

HQ651178.1

NA

ND

ND

[75]

Limonene

ag10

Abies grandis

AF006193.1

NA

ND

+

[61]

Pinene

PT30

Pinus taeda

AF543530.1

Km = 47 ± 9 µM

ND

++

[62]

AG3.18

Abies grandis

U87909.1

Km = 6 µM

ND

+

[61, 76]

Myrcene

amaOc15

Antirrhinum majus

AY195608.1

NA

ND

ND

[60]

ama1e20

Antirrhinum majus

AY195609.1

NA

ND

ND

[60]

AG2.2

Abies grandis

U87908.1

NA

ND

ND

[77]

Linalool

PaTPS-Lin

Picea Abies

AY473623.1

NA

ND

ND

[78]

Sabinene

RlemTPS2

Citrus jambhiri

AB266585.1

NA

ND

ND

[79]

SabS1

Salvia pomifera

DQ785794.1

Km = 7.4 µM

++

++

[63, 64]

Carene

PaJF67

Picea abies

AF461460

NA

ND

ND

[80]

TpsB

Salvia stenophylla

AF527416.1

NA

ND

++

[65]

Thujene

LcTPS2

Licea cubeba

HQ651179.1

NA

ND

ND

[75]

  1. ND, not detectable; +, detectable < 1 mg/L; ++, < 5 mg/L; +++, 5–20 mg/L