Fig. 1
From: Synthetic metabolic pathway for the production of 1-alkenes from lignin-derived molecules
A schematic representation of medium-chain alkene production from ferulate (a) and glucose (b) by A. baylyi ADP1. In A. baylyi ADP1, genes hcaC and vanA,B encode for coenzyme A ligase and vanillate demethylation machinery respectively, responsible for conversion of ferulate to protocatechuate. Protocatechuate is further converted to the central metabolite, acetyl-CoA (coenzyme A), via β-ketoadipate pathway. Acetyl-CoA is also the precursor of fatty acid synthesis. Genes ‘tesA and undA, encoding for thioesterase and decarboxylase, respectively, are heterologously expressed in A. baylyi ADP1 for 1-undecene production. 1-Undecene is a semivolatile hydrocarbon, which can be directly collected from the culture headspace