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Table 2 Descriptions and sources of plasmids used in this study

From: Fermentative production of the unnatural amino acid l-2-aminobutyric acid based on metabolic engineering

Plasmids Description Sources
pTrc99a AmpR, pBR322-origin, trc promoter, 4.2 kb [35, 38]
pCas KanR, Cas9 nuclease expression plasmid, temperature-sensitive origin [39, 40]
pTrc-ilvA* AmpR, ilvA* cloned in the sac I and BamH I site of pTrc99A This study
Gap-ilvA* AmpR, replacement of Trc promoter in pTrc-ilvA* with the Gap promoter This study
Pbs-ilvA* AmpR, replacement of Trc promoter in pTrc-ilvA* with the Pbs promoter This study
pTrc-ilvA* -BleuDH AmpR, BleuDH cloned in the Xba I and Hind III site of pTrc-ilvA* This study
pTrc-ilvA*-leuDH AmpR, leuDH cloned in the Xba I and Hind III site of pTrc-ilvA* This study
pTrc-ilvA*-Trc-leuDH AmpR, leuDH carrying a Trc promoter and RBS cloned in the Xba I and Hind III site of pTrc-ilvA* This study
pTrc-ilvA*-Gap-leuDH AmpR, leuDH carrying a Gap promoter and RBS cloned in the Xba I and Hind III site of pTrc-ilvA* This study
pTrc-ilvA*-Pbs-leuDH AmpR, leuDH carrying a Pbs promoter and RBS cloned in the Xba I and Hind III site of pTrc-ilvA* This study
Gap-ilvA*-leuDH AmpR, replacement of Trc promoter in pTrc-ilvA*-leuDH with the Gap promoter This study
Gap-ilvA*-Trc-leuDH AmpR, leuDH carrying a Trc promoter and RBS cloned in the Xba I and Hind III site of Gap-ilvA* This study
Gap-ilvA*-Gap-leuDH AmpR, leuDH carrying a Gap promoter and RBS cloned in the Xba I and Hind III site of Gap-ilvA* This study
Gap-ilvA*-Pbs-leuDH AmpR, leuDH carrying a Pbs promoter and RBS cloned in the Xba I and Hind III site of Gap-ilvA* This study
Pbs-ilvA*-leuDH AmpR, replacement of Trc promoter in pTrc-ilvA*-leuDH with the Pbs promoter This study
Pbs-ilvA*-Trc-leuDH AmpR, leuDH carrying a Trc promoter and RBS cloned in the Xba I and Hind III site of Pbs-ilvA* This study
Pbs-ilvA*-Gap-leuDH AmpR, leuDH carrying a Gap promoter and RBS cloned in the Xba I and Hind III site of Pbs-ilvA* This study
Pbs-ilvA*-Pbs-leuDH AmpR, leuDH carrying a Pbs promoter and RBS cloned in the Xba I and Hind III site of pbs-ilvA* This study
pTarget-X A plasmid used to transcript sgRNA targeting the particular gene X in genome. X refers to the amino acid biosynthetic pathways This study
  1. Sp spectinomycin, Kan kanamycin, Amp ampicillin, R resistance