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Table 1 Characteristics and sources of bacterial strains used in this study

From: Fermentative production of the unnatural amino acid l-2-aminobutyric acid based on metabolic engineering

Strains Characteristics Sources
E. coli W3110 Coli Genetic Stock Center strain (CGSC) No. 4474 CGSCa
E. coli TOP10 F mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 deoR recA1 araD139 Δ(ara-leu)7697galU galK rpsL endA1 nupG Invitrogenb
E. coli THR W3110 (ΔlacI, thrAC1034T, lysCC1055T, PthrABC::Ptac, ΔlysA, ΔmetA, Δtdh, ΔiclR, Pppc::PTrc) This study
E. coli THR ΔrhtA E. coli THR derivative, ΔrhtA This study
E. coli THR ΔrhtC E. coli THR derivative, ΔrhtC This study
E. coli THR ΔrhtAΔrhtC E. coli THR derivative, ΔrhtArhtC This study
E. coli THR ΔrhtAΔilvIH E. coli THR derivative, ΔrhtAilvIH This study
  1. aInvitrogen, Crop., Carlsbad, CA
  2. bColi Genetic Stock Center