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Table 2 Independent polymorphisms lead to a C-terminal extension of LipY-OVA

From: Optimization of secretion and surface localization of heterologous OVA protein in mycobacteria by using LipY as a carrier

Construct

C-terminal amino acid sequence

Deletion/insertion (bp)

LipY-OVAwt

YPYDVPDYA

–

LipY-OVA1

YPYDVPDYLRYLERPPPRCEPDLVIMS

Δ942T and Δ943G

LipY-OVA3

YPYDVPDYALRYLERPPPRCEPDLVIMS

+ 944C

  1. Depicted is the C-terminal amino acid sequence of LipY-OVAwt containing the HA-tag. Constructs LipY-OVA1 and LipY-OVA3 had independent deletions/insertions (column 3) leading to a similar C-terminal extension after the HA-tag (Column 2, shown in italics). Proline triplet is underlined
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Microbial Cell Factories

ISSN: 1475-2859

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