Table 1 Plasmids used in this study
Plasmid name | Plasmid backbone, promotor, selection marker | Protein product | Reference |
|---|---|---|---|
pSMT3-lipY-OVA | pSMT3, hsp60, hygromycin | Rv3097c aa 1–205, OVA | This study |
pSMT3-lipY-OVA1 | pSMT3, hsp60, hygromycin | Rv3097c aa 1–205 mut 1, OVA mut 1 | This study |
pSMT3-lipY-OVA2 | pSMT3, hsp60, hygromycin | Rv3097c aa 1–205 mut 2, OVA mut 2 | This study |
pSMT3-lipY-OVA3 | pSMT3, hsp60, hygromycin | Rv3097c aa 1–205 mut 3, OVA mut 3 | This study |
pSMT3-lipYwt-OVA1 | pSMT3, hsp60, hygromycin | Rv3097c aa 1–205, OVA mut 1 | This study |
pSMT3-lipYwt-OVA3 | pSMT3, hsp60, hygromycin | Rv3097c aa 1–205, OVA mut 3 | This study |
pSMT3-lipY1-OVAwt | pSMT3, hsp60, hygromycin | Rv3097c mut 1, OVA | This study |
pSMT3-lipY3-OVAwt | pSMT3, hsp60, hygromycin | Rv3097c mut 3, OVA | This study |
pSMT3-lipY-OVAL280Pext | pSMT3, hsp60, hygromycin | Rv3097c aa 1–205, OVA L280P with extension caused by framehsift | This study |
pSMT3-lipY-OVAL280P | pSMT3, hsp60, hygromycin | Rv3097c aa 1–205, OVA L280P | This study |
pSMT3-lipYtub | pSMT3, hsp60, hygromycin | Rv3097c | Daleke et al. [38] |
pSMT3-lipY-Δ100–145 | pSMT3, hsp60, hygromycin | Rv3097c Δ100–145 | This study |
pSMT3-lipY-Δ158–180 | pSMT3, hsp60, hygromycin | Rv3097c Δ158–180 | This study |
pSMT3-lipY-Δ158–205 | pSMT3, hsp60, hygromycin | Rv3097c Δ158–205 | This study |
pSMT3-lipY-Δ191–205 | pSMT3, hsp60, hygromycin | Rv3097c Δ191–205 | This study |
pSMT3-lipY-Δ98–201 | pSMT3, hsp60, hygromycin | Rv3097c Δ98–201 | This study |