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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Optimization of secretion and surface localization of heterologous OVA protein in mycobacteria by using LipY as a carrier

Fig. 6

The effect of linker domain deletions in LipYtub. a Schematic representation of deletions that were made in the linker domain of LipY. b Immunoblot showing whole cell lysate (WCL), genapol pellet (GP), genapol supernatant (GS) and culture filtrate (CF) of M. marinum expressing LipYtub and LipYtub linker domain deletion mutants. Proteins were visualized with anti-HA and anti-GroEL (cytosolic control). Total amount of HA-labeled protein was calculated for each blot separately using ImageJ Gel Analysis tools and the highest score was set to 100%, taking into account all bands in the lane. c Lipase activity was measured on whole cells M. marinum by using DGGR as a substrate. One representative experiment is shown out of three independent experiments that showed similar results

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