Fig. 4
Secretion analysis of LipY-OVA L280P mutation. Immunoblot of whole cell lysate (WCL), genapol pellet (GP), genapol supernatant (GS) and culture filtrate (CF) of M. marinum WT, LipY-OVAwt, LipY-OVA3, LipYwt-OVA1, LipY-OVAL280Pext and LipY-OVAL280P. Proteins were visualized by anti-HA-tag and anti-GroEL2 (cytosolic control). Total amount of HA-labeled protein was calculated for each blot separately using ImageJ Gel Analysis tools and the highest score was set to 100%, taking into account all bands in the lane