Fig. 3From: Optimization of secretion and surface localization of heterologous OVA protein in mycobacteria by using LipY as a carrierSecretion analysis of LipY-OVA mutants. Immunoblots of whole cell lysate (WCL), genapol pellet (GP), genapol supernatant (GS) and culture filtrate (CF) of M. marinum WT, LipYtub-, LipY-OVAwt, LipY-OVA1, LipY-OVA2 and LipY-OVA3. Proteins were visualized with anti-HA and anti-GroEL2 (cytosolic control). Total amount of HA-labeled protein was calculated for each blot separately using ImageJ Gel Analysis tools and the highest score was set to 100%, taking into account all bands in the laneBack to article page