Fig. 1
ESX-5 dependent secretion of LipY-OVA in M. marinum. a The PE domain and linker domain of LipYtub are fused to OVA (amino acid 259-357 of ovalbumin) and a C-terminal HA-tag. b Immunoblot of secretion analysis of LipY-OVAwt in M. marinum WT and the ESX-5 mutant espG5::tn (7C1). WCL Whole cell lysate, GP genapol pellet, GS, surface-enriched protein fraction genapol supernatant, CF culture filtrate are shown. Blot was stained with antibodies directed against HA and GroEL2 (cytosolic control)