Pectinase secreted by psychrotolerant fungi: identification, molecular characterization and heterologous expression of a cold-active polygalacturonase from Tetracladium sp.

Table 2 Expression, at the transcript level, of putative pectate lyase- and polygalacturonase-encoding genes from Tetracladium sp

Putative	CDS	Expression (FPKM)		P/G^a
Putative	CDS	YM-G	YM-P
PL	scaffold_4.g450.t1	3.6	0.8	− 4
PL	scaffold_5.g260.t1	22.9	6.5	− 4
PL	scaffold_4.g243.t1	2.2	0.8	− 3
PL	scaffold_2.g1041.t1	2.4	0.9	− 3
PL	scaffold_0.g2020.t1	5.6	3.3	− 2
PL	scaffold_1.g759.t1	6.5	4.2	− 2
PL	scaffold_1.g52.t1	29.9	22.0	− 1
PL	scaffold_0.g2289.t1	1.1	0.8	− 1
PL	scaffold_11.g858.t1	26.0	45.2	2
PL	scaffold_7.g463.t1	7.0	15.0	2
PL	scaffold_3.g58.t1	18.0	60.9	3
PL	scaffold_0.g2293.t1	5.5	19.7	4
PG	scaffold_3.g273.t1	1.6	11.8	7
PG	scaffold_3.g424.t1	19.1	243.4	13
PL	scaffold_2.g849.t1	2.6	74.9	28
PG	scaffold_3.g812.t1	0.6	66.3	112
PL	scaffold_3.g812.t1	0.6	66.3	112
PG	scaffold_0.g606.t1	3.9	1012.9	258
PG	scaffold_3.g1104.t1	1.0	291.1	288
PG	scaffold_10.g413.t1	0.1	1762.5	12,228
PG	scaffold_6.g590.t1	0.0	566.6	12,360

Expression in medium supplemented with glucose (YM-G) or pectin (YM-P). PL pectate lyase, PG polygalacturonase. ^aExpression fold changes between cultures in YM-P (P) and YM-G (G) media

ISSN: 1475-2859