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Table 1 Microorganism, plasmids and oligonucleotides used in this work

From: Pectinase secreted by psychrotolerant fungi: identification, molecular characterization and heterologous expression of a cold-active polygalacturonase from Tetracladium sp.

Microorganism/plasmid/oligonucleotide

Description

References

Yeast/yeast-like

 Tetracladium sp.

30 °Ca

[22]

 Dioszegia sp.

15 °Ca

[22]

 Rhodotorula glacialis (Phenoliferia glacialis) (T8Rg)

22 °Ca

[22]

 Leuconeurospora sp. T17Cd1

15 °Ca

[22]

 Leucosporidiella fragaria (Leucosporidium fragarium)

22 °Ca

[22]

 Metschnikowia sp.

10 °Ca

[22]

 Mrakia psychrophila

10 °Ca

[22]

 Mrakia robertii

15 °Ca

[22]

 Rhodotorula glacialis (Phenoliferia glacialis) (T11Rs)

22 °Ca

[22]

 Rhodotorula (Cystobasidium) laryngis

30 °Ca

[22]

 Sporidiobolus salmonicolor

22 °Ca

[22]

 Wickerhamomyces anomalus

30 °Ca

[22]

 PichiaPink strain 4

P. pastoris strain: ade2, prb1, pep4

Invitrogen

 PichiaPink strain 2

P. pastoris strain: ade2, pep4

Invitrogen

Bacteria

 Escherichia coli Top10

F-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 Δ lacX74 recA1 araD139 Δ(araleu)7697 galU galK rpsL (StrR) endA1 nupG

Thermo Fisher

Plasmids

 pPinkα-HC

P. pastoris integrating vector for high-copy expression of a secreted protein

Invitrogen

Oligonucleotides

 Pectfw

5′-GCACCTACAGTCTCATCATTG-3′

This work

 Pectrev

5′-GCAGGAAGCAGGGGATGGGAA-3′

This work

  1. aOptimal temperature for growth. The current taxonomic classification is given in parentheses