Pectinase secreted by psychrotolerant fungi: identification, molecular characterization and heterologous expression of a cold-active polygalacturonase from Tetracladium sp.

Table 1 Microorganism, plasmids and oligonucleotides used in this work

Microorganism/plasmid/oligonucleotide	Description	References
Yeast/yeast-like
Tetracladium sp.	30 °C^a	[22]
Dioszegia sp.	15 °C^a	[22]
Rhodotorula glacialis (Phenoliferia glacialis) (T8Rg)	22 °C^a	[22]
Leuconeurospora sp. T17Cd1	15 °C^a	[22]
Leucosporidiella fragaria (Leucosporidium fragarium)	22 °C^a	[22]
Metschnikowia sp.	10 °C^a	[22]
Mrakia psychrophila	10 °C^a	[22]
Mrakia robertii	15 °C^a	[22]
Rhodotorula glacialis (Phenoliferia glacialis) (T11Rs)	22 °C^a	[22]
Rhodotorula (Cystobasidium) laryngis	30 °C^a	[22]
Sporidiobolus salmonicolor	22 °C^a	[22]
Wickerhamomyces anomalus	30 °C^a	[22]
PichiaPink strain 4	P. pastoris strain: ade2, prb1, pep4	Invitrogen
PichiaPink strain 2	P. pastoris strain: ade2, pep4	Invitrogen
Bacteria
Escherichia coli Top10	F-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 Δ lacX74 recA1 araD139 Δ(araleu)7697 galU galK rpsL (StrR) endA1 nupG	Thermo Fisher
Plasmids
pPinkα-HC	P. pastoris integrating vector for high-copy expression of a secreted protein	Invitrogen
Oligonucleotides
Pectfw	5′-GCACCTACAGTCTCATCATTG-3′	This work
Pectrev	5′-GCAGGAAGCAGGGGATGGGAA-3′	This work

^aOptimal temperature for growth. The current taxonomic classification is given in parentheses

ISSN: 1475-2859