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Table 1 Microorganism, plasmids and oligonucleotides used in this work

From: Pectinase secreted by psychrotolerant fungi: identification, molecular characterization and heterologous expression of a cold-active polygalacturonase from Tetracladium sp.

Microorganism/plasmid/oligonucleotide Description References
Yeast/yeast-like
 Tetracladium sp. 30 °Ca [22]
 Dioszegia sp. 15 °Ca [22]
 Rhodotorula glacialis (Phenoliferia glacialis) (T8Rg) 22 °Ca [22]
 Leuconeurospora sp. T17Cd1 15 °Ca [22]
 Leucosporidiella fragaria (Leucosporidium fragarium) 22 °Ca [22]
 Metschnikowia sp. 10 °Ca [22]
 Mrakia psychrophila 10 °Ca [22]
 Mrakia robertii 15 °Ca [22]
 Rhodotorula glacialis (Phenoliferia glacialis) (T11Rs) 22 °Ca [22]
 Rhodotorula (Cystobasidium) laryngis 30 °Ca [22]
 Sporidiobolus salmonicolor 22 °Ca [22]
 Wickerhamomyces anomalus 30 °Ca [22]
 PichiaPink strain 4 P. pastoris strain: ade2, prb1, pep4 Invitrogen
 PichiaPink strain 2 P. pastoris strain: ade2, pep4 Invitrogen
Bacteria
 Escherichia coli Top10 F-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 Δ lacX74 recA1 araD139 Δ(araleu)7697 galU galK rpsL (StrR) endA1 nupG Thermo Fisher
Plasmids
 pPinkα-HC P. pastoris integrating vector for high-copy expression of a secreted protein Invitrogen
Oligonucleotides
 Pectfw 5′-GCACCTACAGTCTCATCATTG-3′ This work
 Pectrev 5′-GCAGGAAGCAGGGGATGGGAA-3′ This work
  1. aOptimal temperature for growth. The current taxonomic classification is given in parentheses