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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Pectinase secreted by psychrotolerant fungi: identification, molecular characterization and heterologous expression of a cold-active polygalacturonase from Tetracladium sp.

Fig. 6

Characterization of the recombinant TPG1. a Analysis by PCR of a recombinant clone (lane 2) in which an amplicon with a size expected for synthetic TPG1 is observed (arrow); Lane 1, DNA ladder. b SDS-PAGE. The arrow indicates the protein band corresponding to the recombinant TPG1; M, molecular marker. c Pectinase activity of TPG1 and a commercial polygalacturonase, evaluated at pH 3.0 and 15 °C using pectin or must as substrate. Data shown are the average values of three independent enzymatic assays

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Microbial Cell Factories

ISSN: 1475-2859

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