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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Pectinase secreted by psychrotolerant fungi: identification, molecular characterization and heterologous expression of a cold-active polygalacturonase from Tetracladium sp.

Fig. 3

Protein purification by ionic exchange chromatography. Purification profile of the 80% ammonium sulfate fraction from Tetracladium sp. culture supernatant is shown. A NaCl gradient from 3 to 195 mM was used as the mobile phase (discontinuous line), and protein presence was detected by absorbance at 280 nm of the fractions (continuous line). Pectinase activity in each fraction was determined (dotted line), and protein content was evaluated by SDS-PAGE (inserted gel photo). SDS-PAGE lanes coincide with the corresponding fraction in the chromatogram. The arrow indicates the probable pectinase enzyme. M, protein marker; E, sample of 80% ammonium sulfate

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Microbial Cell Factories

ISSN: 1475-2859

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