Strain/plasmid | Description/function | References |
---|---|---|
Strains | ||
 C. glutamicum ATCC13032 | Wild-type | ATCC |
 JS34 | C. glutamicum inserted with attB site on the chromosome | [33] |
 JS45-A, B, C, D, E | JS34 site-specific integrated the lacSZ operon, KanR | This study |
 JS46 | Marker excised via lox66–lox71 recombination from JS45-E | This study |
 JS86-A, B, C, D | JC114-E site-specific integrated the galMKTE operon, KanR | This study |
 JS93 | Marker excised via lox66–lox71 recombination from JS86-D | This study |
 JS95 | Evolved JS93 | This study |
 JS99 | JS95Δldh | This study |
 JS112 | JS99Δppc | This study |
 JS122 | JS112 bearing pJS115, SpecR | This study |
 S. thermophilus | Source of the lacSZ operon | [35] |
 L. lactis MG 1363 | Source of the galMKTE operon | [53] |
 E. coli TOP 10 | Transformation host | Lab stock |
Plasmids | ||
 pK18mobsacB | Used for deleting ldh and ppc genes, KanR | [50] |
 pJS31 | Used for site-specific integration, KanR | [33] |
 pAL347 | E. coli–C. glutamicum expression shutter vector, SpecR | [57] |
 pJS115 | A derivative from pAL347 for ethanol producing, SpecR | This study |