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Table 2 Strains and plasmids

From: Development of a novel, robust and cost-efficient process for valorizing dairy waste exemplified by ethanol production

Strain/plasmid

Description/function

References

Strains

 C. glutamicum ATCC13032

Wild-type

ATCC

 JS34

C. glutamicum inserted with attB site on the chromosome

[33]

 JS45-A, B, C, D, E

JS34 site-specific integrated the lacSZ operon, KanR

This study

 JS46

Marker excised via lox66–lox71 recombination from JS45-E

This study

 JS86-A, B, C, D

JC114-E site-specific integrated the galMKTE operon, KanR

This study

 JS93

Marker excised via lox66–lox71 recombination from JS86-D

This study

 JS95

Evolved JS93

This study

 JS99

JS95Δldh

This study

 JS112

JS99Δppc

This study

 JS122

JS112 bearing pJS115, SpecR

This study

 S. thermophilus

Source of the lacSZ operon

[35]

 L. lactis MG 1363

Source of the galMKTE operon

[53]

 E. coli TOP 10

Transformation host

Lab stock

Plasmids

 pK18mobsacB

Used for deleting ldh and ppc genes, KanR

[50]

 pJS31

Used for site-specific integration, KanR

[33]

 pAL347

E. coli–C. glutamicum expression shutter vector, SpecR

[57]

 pJS115

A derivative from pAL347 for ethanol producing, SpecR

This study