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Table 1 ACAT, AACR, and PHAP activities in recombinant R. sphaeroides HJ cells

From: Disruption of poly (3-hydroxyalkanoate) depolymerase gene and overexpression of three poly (3-hydroxybutyrate) biosynthetic genes improve poly (3-hydroxybutyrate) production from nitrogen rich medium by Rhodobacter sphaeroides

Strain

Enzyme activity (U/g-protein)

ACAT

AACR

PHAP

HJΔphaZ (pLP-1.2)

8.2 ± 0.3

5.3 ± 0.2

0.7 ± 0.1

HJΔphaZ (phaA1)

22.5 ± 0.6

–

–

HJΔphaZ (phaA2)

18.6 ± 0.8

–

–

HJΔphaZ (phaA3)

19.2 ± 0.5

–

–

HJΔphaZ (phaA4)

19.1 ± 0.5

–

–

HJΔphaZ (phaB1)

–

19.5 ± 1.2

–

HJΔphaZ (phaB2)

–

21.0 ± 1.7

–

HJΔphaZ (phaC1)

–

–

4.8 ± 0.1

HJΔphaZ (phaC2)

–

–

3.4 ± 0.2

HJΔphaZ (phaA3/phaB2/phaC1)

20.5 ± 1.5

16.0 ± 0.5

3.5 ± 0.3

  1. The recombinant R. sphaeroides HJ cells were anaerobically grown in AAY medium containing 10 mM AS at 30 °C for 3 days under illumination at 8.2 W m−2
  2. –, The data were not measured
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Microbial Cell Factories

ISSN: 1475-2859

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