Fig. 2From: Engineering of leucine-responsive regulatory protein improves spiramycin and bitespiramycin biosynthesisThe predicted domains and three-dimensional (3D) structure of SSP_Lrp. a 3D structure and SDS-PAGE detection of His6-tagged SSP_Lrp protein. b EMSAs of DNA binding of Lrp to Pbsm42, Pbsm23, and Pist-acyB2. C1: Pbsm42-SSP_Lrp complex; C2: Pbsm23-SSP_Lrp complex; C3: Pist-acyB2-SSP_Lrp complex; N: nonspecific control DNA. *P < 0.05, **P < 0.01Back to article page