Bacteria | M9 medium without glucose supplemented with | Number of experiments | Duration | pH of the culture beforea | Density of the culture OD600nm before | Non-gaseous fermentation products | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1st passage | 2nd passage | End sampling | 1st passage | 2nd passage | End sampling | ||||||||
2nd day | 3rd day | 2nd day | 3rd day | 2nd day | 3rd day | ||||||||
Microbial community from a dark fermentation bioreactor | Molasses | 3 | 9 days, 2 passages (every 3 days) | 4.6–4.8 | 4.7–4.8 | 4.6–4.9 | 0.5–0.6 | 2.7–3.1 | 0.4–0.5 | 2.3–3.1 | 0.5–0.6 | 2.9–3.0 | Figure 1a |
Molasses and sodium lactate | 3 | 4.8–5.5 | 4.8–5.5 | 4.6–5.0 | 0.7–0.8 | 2.0–2.6 | 0.6–0.8 | 1.8–3.2 | 0.7–0.8 | 2.4–2.6 | |||
Molasses, sodium lactate and sodium acetate | 2 | 4.7–5.0 | 4.6–5.1 | 4.7–4.8 | 0.5–0.7 | 1.5–3.1 | 0.5–0.6 | 1.6–2.3 | 0.5–0.6 | 2.5–2.6 | |||
6.5–6.7 | 6.7–6.8 | 6.5–6.7 | 0.2–0.3 | 0.9–1.0 | 0.2–0.3 | 0.8–1.0 | 0.2–0.3 | 0.8–1.0 | Figure 1b |
Bacteria | M9 medium without glucose supplemented with | Number of experiments | Duration | pH of the culture after | Density of the culture OD600nm after | Non-gaseous fermentation products | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
3 days | 6 days | 9 days | 3 days | 6 days | 9 days | ||||||||
C. butyricum | Sodium lactate, sodium acetate and yeast extract | 3 | 9 days, no passage | 6.8–6.9 | 6.8–7.0 | 6.8–7.1 | ~ 0.20 | ~ 0.6 | ~ 0.7 | Figure 1c |