Table 2 Plasmids and strains used in this study
From: Establishment of BmoR-based biosensor to screen isobutanol overproducer
| Â | Description | Source |
|---|---|---|
Plasmids | ||
 pUC19 | Plac-lacZα; colE1; ampr | TransGen Biotech |
 pYK | PLlacO1; LacI; colA; kanr | [16] |
 pSA74 | PLlacO1; pSC101*; cmr | [36] |
 pSA65 | PLlacO1-kivd-adhA; colE1; ampr | [8] |
 pSA69 | PLlacO1-alsS-ilvC-ilvD; p15A; kanr | [8] |
 pYH1 | PbmoR-bmoR; Pbmo-gfp; colE1; ampr | This study |
 pYH2 | PbmoR-bmoR; Pbmo-lacZα; colE1; ampr | This study |
 pYH3 | PbmoR-bmoR; Pbmo-lacZα; pSC101*; cmr | This study |
 pYH4 | PbmoR-bmoR; Pbmo-lacZα; PLlacO1-kivd-adhA; colE1; ampr | This study |
 pYH5 | PbmoR-bmoR; Pbmo-lacZα; colA; cmr | This study |
 pYH9 | PbmoR-bmoR; Pbmo-gfp; PLlacO1-kivd-adhA; colE1; ampr | This study |
 pYH10 | PbmoR-bmoR; Pbmo-gfp; colA; cmr | This study |
 pYH11 | PbmoR-bmoR; Pbmo-gfp; pSC101*; cmr | This study |
Strains | ||
 XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqZΔM15 Tn10 (Tetr)] | [8] |
 BW25113 (F′) | rrnBT14ΔlacZWJ16hsdR514ΔaraBADAH33 ΔrhaBADLD78F′ [traD36 proABlacIqZΔM15 Tn10(Tet r )] | [8] |
 YH1 | BW25113 (F′) with pYH1 | This study |
 YH2 | BW25113 (F′) with pYH2 | This study |
 YHS1 | BW25113 (F′) with pSA69 and pYH4 | This study |
 YHS2 | BW25113 (F′) with pSA69, pSA65 and pYH5 | This study |
 YHS3 | BW25113 (F′) with pSA69, pSA65 and pYH3 | This study |
 YHS4 | BW25113 (F′) with pSA65 and pYH5 | This study |
 YHS5 | BW25113 (F′) with pSA65 and pYH10 | This study |
 YHS6 | Mutant 10 with pSA69 | This study |