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Table 2 Plasmids and strains used in this study

From: Establishment of BmoR-based biosensor to screen isobutanol overproducer

  Description Source
Plasmids
 pUC19 Plac-lacZα; colE1; ampr TransGen Biotech
 pYK PLlacO1; LacI; colA; kanr [16]
 pSA74 PLlacO1; pSC101*; cmr [36]
 pSA65 PLlacO1-kivd-adhA; colE1; ampr [8]
 pSA69 PLlacO1-alsS-ilvC-ilvD; p15A; kanr [8]
 pYH1 PbmoR-bmoR; Pbmo-gfp; colE1; ampr This study
 pYH2 PbmoR-bmoR; Pbmo-lacZα; colE1; ampr This study
 pYH3 PbmoR-bmoR; Pbmo-lacZα; pSC101*; cmr This study
 pYH4 PbmoR-bmoR; Pbmo-lacZα; PLlacO1-kivd-adhA; colE1; ampr This study
 pYH5 PbmoR-bmoR; Pbmo-lacZα; colA; cmr This study
 pYH9 PbmoR-bmoR; Pbmo-gfp; PLlacO1-kivd-adhA; colE1; ampr This study
 pYH10 PbmoR-bmoR; Pbmo-gfp; colA; cmr This study
 pYH11 PbmoR-bmoR; Pbmo-gfp; pSC101*; cmr This study
Strains
 XL1-Blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqZΔM15 Tn10 (Tetr)] [8]
 BW25113 (F′) rrnBT14ΔlacZWJ16hsdR514ΔaraBADAH33 ΔrhaBADLD78F′ [traD36 proABlacIqZΔM15 Tn10(Tet r )] [8]
 YH1 BW25113 (F′) with pYH1 This study
 YH2 BW25113 (F′) with pYH2 This study
 YHS1 BW25113 (F′) with pSA69 and pYH4 This study
 YHS2 BW25113 (F′) with pSA69, pSA65 and pYH5 This study
 YHS3 BW25113 (F′) with pSA69, pSA65 and pYH3 This study
 YHS4 BW25113 (F′) with pSA65 and pYH5 This study
 YHS5 BW25113 (F′) with pSA65 and pYH10 This study
 YHS6 Mutant 10 with pSA69 This study